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Home / Kits and assays / TEAC Assay
Kits and assays

TEAC Assay

The TEAC assay is used to determine whether the tested sample has antioxidant properties. In this assay, a substance whose antioxidant activity has to be determined is compared with a known antioxidant: Trolox. A radical solution, ABTS•+ radicals, is prepared by adding ABTS and K2O8S2 and letting is react for 12 to 16 hours. ABTS•+ radical cations absorb light at a wavelength of 734 nm. This absorption decreases as soon as an antioxidant is added, forming a colorless product (see figure below). The extent to which this absorption decreases determines the antioxidant capacity of a given sample, which in this assay kit is compared to Trolox.

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    Description

    The trolox equivalent antioxidant capacity (TEAC) assay originated as a straightforward method for assessing total antioxidant capacity (TAC) without the need for complex procedures. This assay evaluates the capability of antioxidants to counteract the stable radical cation ABTS+ (2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)), a blue-green chromophore with peak absorption at 734 nm, which diminishes in intensity when antioxidants are present. Antioxidants can deactivate the ABTS+ radical cation produced from ABTS either through direct electron donation, thereby reducing it, or by intercepting radicals through hydrogen atom donation. The relative contribution of these mechanisms is typically influenced by the structure of the antioxidant and the pH of the solution.

    Contents of the kit

    • · Trolox Standard for stock solution, in amber vial
    • · ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)); in amber vial
    • · Potassium persulfate; in amber vial

    Additional information

    Amount

    1 kit

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    Our products are used by researchers and diagnostic companies all over the world. We ship our products with FedEx to your country of choice.

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    We produce large batches so you are assured of material from the same batch over a longer period of time. You can even make a reservation for material from a specific lot.

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    We have a dedicated team to answer all of your questions. Satisfied customers is our number one priority.

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    Kits and assays

    TEAC Assay

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    • Our quality
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    • Admiraal de Ruyterlaan 5
    • 9726 GN Groningen
    • The Netherlands
    • info@citeq.com
    • +31 50 3137464
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