Cysteine protease kit
Proteases are a type of enzyme that can break peptide bonds of proteins via hydrolysis. They are well known therapeutic targets, such as proteases from the SARS-CoV-2 virus [1] or certain allergens like Der p1. A cysteine protease contain a cysteine on the active site. The most well-known cysteine protease is the protease papain, member of the papain-like protease (PLP).
Description
By making a standard curve based on known units of the standard papain (EC 3.4.22.2), the amount of units in a solution can be interpolated. A unit is the amount of enzyme needed to transform 1 µmol of substrate per minute. Papain-like proteases can cleave the substrate, releasing p-nitroaniline (pNA) in the process, which produces a pale yellow color. The reaction is subsequently quantified measuring the optical density at 405 nm.
Contents of the kit
■ Papain standard (1 vial)
■ Substrate concentrate (1 vial)
■ Stabilizing buffer (1 vial)
■ Certificate of Analysis
■ User manual with plate layout
Additional information
| Amount | 1 kit |
|---|
