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Home / Kits and assays / Haba assay kit
Kits and assays

Haba assay kit

This assay is versatile and can be used for different means, such as detection of biotin in food or products and the quantification of biotin molecules to antibodies. The assay is quick and easy to perform and gives a proper estimate of the amount of biotin present in your samples. To ensure the validity of your reactions, a positive control is provided with this kit. It is the Citeq 02.01.88 D. pteronyssinus biotinylated extract, which can be dissolved easily in a buffer of your choice, making this kit a quick and easy assay with minimal steps. No fluorescence readers are required, making this assay accessible, as only a plate reader will be needed.

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    • Description
    • Additional information

    Description

    The connection between biotin and avidin and/or streptavidin is one of the strongest non-covalent bonds currently known (Ka = 1015 M-1). This bond can withstand extremes in pH and solvents. Biotin is a small molecule (244 Da) and can bind avidin even when attached to other proteins1, without hindering the activity of these proteins. Most proteins can be biotinylated, allowing for a simple way to connect two elements together. This is utilized in many research applications such as ELISA or immunohistochemistry as to link two antibodies together, allowing for detection of specific proteins. Even tough biotin is a small molecule, it can sterically hinder the protein if too many biotin molecules are added to the primary protein. Therefore it is important to know how many biotin molecules are attached to the main molecule in order to predict its’ functionality.

    In order to check the amount of biotin molecules attached to a protein, one can perform an HABA assay. 4´-hydroxyazobenzene-2-carboxylic acid (HABA) has a weak affinity to avidin and emits an orange/red color when hydrolyzed. This hydrolyzed HABA can be detected at 500 nm. The affinity between biotin and avidine is much stronger however, so when a sample containing biotin is added, this biotin attaches to the avidin, displacing the HABA in the process. The HABA reverts back to its original state and the absorbance at 500nm decreases proportionately, allowing for determination of the amount of biotin in the sample.

    Contents of the kit

    • 1 bottle of HABA-avidin premix (for 10 tests)
    • 1 vial of Citeq biotin standard (30kDa)
    • Manual

    Not provided but necessary: buffers (such as PBS), a cuvet (1mL) or microtiterplate (96-wells), an absorbance reader, pipets (200 µL) with appropriate tips.

     

    Additional information

    Amount

    for 10 tests

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    • Biotinylated material

      Biotinylated D. pteronyssinus extract


    Freeze-dried

    Our material will be delivered in freeze-dried (lyophilized) form in different sizes so you always have the right amount available and you are assured of stable material.

    Defined

    Because of our quality system we produce high quality allergen extracts. Our products will be delivered with a certificate that specifies the amount of allergens, level of endotoxin and much more.

    Customization

    On request we can customize products for you. Please contact us for any specific requests or collaborations.

    Woldwide delivery

    Our products are used by researchers and diagnostic companies all over the world. We ship our products with FedEx to your country of choice.

    Large batches

    We produce large batches so you are assured of material from the same batch over a longer period of time. You can even make a reservation for material from a specific lot.

    100% service

    We have a dedicated team to answer all of your questions. Satisfied customers is our number one priority.

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    Kits and assays

    Haba assay kit

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    Other
    • Our quality
      • Defined material
      • Stability extracts
      • Large batches
      • Freeze-dried form
      • Content uniformity
      • Our services
        • Custom products
        • Mite identification
    • Support & Information
      • FAQ
      • Extra information
    • News
    • Contact
    • Privacy Statement
    Contact
    • Citeq BV
    • Admiraal de Ruyterlaan 5
    • 9726 GN Groningen
    • The Netherlands
    • info@citeq.com
    • +31 50 3137464
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