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Home / Kits and assays / Determination of successful sensitization of mice using biotinylated D. pteronyssinus
Antibodies
Antibodies

Determination of successful sensitization of mice using biotinylated D. pteronyssinus



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  • Freeze-dried
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    • Description

    Description

    Determination of successful sensitization of mice using biotinylated D. pteronyssinus

    Our polyclonal anti-mouse antibodies can be used together with our biotinylated HDM allergen products to quantify HDM allergen specific antibodies in mouse serum using an ELISA method. This kit contains the IgE/ IgG1/ IgG2a antibodies and biotinylated D. pteronyssinus HDM.

    Quantification of antigen specific serum antibodies

    The antibody can be coated on an ELISA plate and act as a capture antibody to immobilize serum IgE/ IgG1/ IgG2a antibodies. In the next step biotinylated HDM allergens are added to facilitate the detection of HDM allergen specific antibodies of the IgE/ IgG1/ IgG2a isotype. Using a reference serum or by comparing multiple sera the HDM allergen specific antibodies can be (relatively) quantified.

    A perfect way for researchers, who are working on allergic asthma research with mouse models, to determine whether immunizations of mice with house dust mite (HDM) have been successful by looking at the amount of specific antibodies against HDM proteins in the serum of mice.

    The specificity of the captured antibodies for HDM must be tested with biotinylated HDM or the biotinylated major allergens like biotinylated Der p 1 or biotinylated Der p 2. The antibodies and biotinylated products are available as separate products but are also available in a handy kit which contains the anti-mouse IgE/ anti-mouse IgG1/ anti-mouse IgG2A and biotinylated HDM extract (or biotinylated Der p 1/ biotinylated Der p 2). For more information about biotinylation we have a page which describes the protocol for the biotinylation products and antibodies.

    Content of the kit

    • 1 x anti-mouse IgE (sufficient for coating  1 x 96-well plate)
    • 1 x anti-mouse IgG1 (sufficient for coating  1 x 96-well plate)
    • 1 x anti-mouse IgG2A (sufficient for coating  1 x 96-well plate)
    • You can choose from the following biotinylated products:
      • Biotinylated D. pteronyssinus (HDM) extract
      • Biotinylated Der p 1
      • Biotinylated Der p 2

    This kit does not include the standard and reference mouse serum. Both can be delivered on request, please contact us for more information.

    Pricing and orderinformation

    Our products are available for worldwide shipping and are sent with FedEx. Orders are shipped within 24 hours after receipt of your order. For more information and pricing please send us an e-mail or give us a call. We will respond as soon as possible.

    You can send us your request on our e-mail address info@citeq.com, give us a call +31(0)50-3137464 or fill in our contact form.


    Freeze-dried

    Our material will be delivered in freeze-dried (lyophilized) form in different sizes so you always have the right amount available and you are assured of stable material.

    Defined

    Because of our quality system we produce high quality allergen extracts. Our products will be delivered with a certificate that specifies the amount of allergens, level of endotoxin and much more.

    Customization

    On request we can customize products for you. Please contact us for any specific requests or collaborations.

    Woldwide delivery

    Our products are used by researchers and diagnostic companies all over the world. We ship our products with FedEx to your country of choice.

    Large batches

    We produce large batches so you are assured of material from the same batch over a longer period of time. You can even make a reservation for material from a specific lot.

    100% service

    We have a dedicated team to answer all of your questions. Satisfied customers is our number one priority.

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    • IgE mouse antibody
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    Antibodies
    Antibodies

    Determination of successful sensitization of mice using biotinylated D. pteronyssinus



    Add to request
    This product is already in quote request list
    Browse the list
    Product Categories
    • House dust mites1111 products
    • Venoms22 products
    • Cockroach33 products
    • Kits and assays55 products
    • Storage mites44 products
    • Pollen Extracts1010 products
    • Molds (Fungi)44 products
    • Food Allergens99 products
    • Animals22 products
    • Antibodies44 products
    • Biotinylated material1010 products
    • Excrements22 products
    Other
    • Our quality
      • Defined material
      • Stability extracts
      • Large batches
      • Freeze-dried form
      • Content uniformity
      • Our services
        • Custom products
        • Mite identification
    • Support & Information
      • FAQ
      • Extra information
    • News
    • Contact
    • Privacy Statement
    Contact
    • Citeq BV
    • Admiraal de Ruyterlaan 5
    • 9726 GN Groningen
    • The Netherlands
    • info@citeq.com
    • +31 50 3137464
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    • Terms & Conditions