Polyclonal anti-mouse IgE antibody
Description
Polyclonal anti-mouse IgE antibody
The affinity purified goat anti-mouse IgE specifically reacts with the IgE heavy chain and not with IgG, IgA, IgM or IgD. It is suitable for use as capture or detection antibody in various immunoassays.
- Product name: Anti-Mouse IgE antibody
- Form: Freeze-dried (lyophilized)
- Size: 133 µg, sufficient for coating one 96-well plate
- Reactivity: Mouse
- Host: Goat
- Antibody type: Secondary antibody
- Specificity/ target: IgE
- Clonality: Polyclonal
- Isotype: IgG
- Purity: Antigen affinity purified
- Suitable for: ELISA, Immunoelectrophoresis, Western Blotting
- Please reconstitute with 1 ml, dilute this 1 ml to 10 ml. Optimal dilutions/ concentrations should be determined by the end user.
Quantification of antigen specific serum antibodies
Together with our biotinylated HDM allergen products, this antibody can be used to quantify HDM allergen specific IgE antibodies in mouse serum using an ELISA method. The antibody can be coated on an ELISA plate and act as a capture antibody to immobilize serum IgE antibodies. In the next step biotinylated HDM allergens are added to facilitate the detection of HDM allergen specific antibodies of the IgE isotype. Using a reference serum or by comparing multiple sera the HDM allergen specific antibodies can be (relatively) quantified.
A perfect way for researchers, who are working on allergic asthma research with mouse models, to determine whether immunizations of mice with house dust mite (HDM) have been successful by looking at the amount of specific antibodies against HDM proteins in the serum of mice. For more information about biotinylation we have a page which describes the protocol for the biotinylation products and antibodies.
Anti-mouse antibodies
- Tested with HDM immunized serum
- Freeze-dried (lyophilized) material
- Long term storage e.g. at – 20°C
- Every buffer applicable to dissolve the antibodies
- Does not contain any salts
- Ready to use in your desired buffer
- Polyclonal antibodies
- Broader affinity
- Part of a group of related antibodies to be used in this application.
- Reference mouse serum available on request
Antibodies and biotinylation kit
The specificity of the captured antibodies for HDM must be tested with biotinylated HDM or the biotinylated major allergens like biotinylated Der p 1 or biotinylated Der p 2. The antibodies and biotinylated products are available as separate products but are also available in a handy kit which contains the anti-mouse IgE/ anti-mouse IgG1/ anti-mouse IgG2A and biotinylated HDM (or biotinylated Der p 1/ biotinylated Der p 2).
Freeze-dried
Our material will be delivered in freeze-dried (lyophilized) form in different sizes so you always have the right amount available and you are assured of stable material.
Defined
Because of our quality system we produce high quality allergen extracts. Our products will be delivered with a certificate that specifies the amount of allergens, level of endotoxin and much more.
Customization
On request we can customize products for you. Please contact us for any specific requests or collaborations.
Woldwide delivery
Our products are used by researchers and diagnostic companies all over the world. We ship our products with FedEx to your country of choice.
Large batches
We produce large batches so you are assured of material from the same batch over a longer period of time. You can even make a reservation for material from a specific lot.
