Biotinylated D. farinae extract
Biotinylated Dermatophagoides farinae extract. One vial of biotinylated D. farinae extract is sufficient for coating of two 96 Microwell plates.
The unique biotinylated D. farinae extract was developed especially for research and diagnostic groups. Biotinylated D. farinae extract can be used to detect specific antibodies in the serum of animal models and/or patients. For example, in animal models, detecting specific D. farinae antibodies can show that the animal is correctly immunized, or that the animal reacts to the treatment (an increase or decrease in levels of specific antibodies). When specific Df-antibodies are detected in patients, it shows high levels of specific Df-antibodies, which indicate that the patient has a house dust mite allergy.
During biotinylation, biotin is attached to macromolecules or proteins. Biotinylated proteins can be used to detect and/or purify proteins due to the high specificity of biotin-avidin interaction. Citeq has developed biotinylated proteins related to house dust mites. These biotinylated proteins include D. pteronyssinus (HDM) extract and the naturally purified Der p 1 protein and Der p 2 protein. Biotinylated proteins can be applied in many protein assays, including western blot analysis, fluorescence-activated cell sorting (FACS)/flow cytometry, and enzyme-linked immunosorbent assay (ELISA).
One vial of biotinylated HDM extract is sufficient for coating of two 96 Microwell plates. On this page you can read more about biotinylation and the protocol for our biotinylated proteins.