The mycelium is mixed with an extraction buffer. Our effective component in the mycelium extraction is phenol, in a concentration of 1 %. We mix the buffer with the mycelium and filtrate this with our last filter is a 0.45 µm filter. We dialysed the filtrated liquid with a volatile buffer, so after freeze drying a minimum amount of salts is available. With this you can use every buffer you prefer in reconstitution the freeze dried product.