How do you liquedify the media?
We cultivate our moulds in a liquid media. In this media we inoculate the mold. During 4- 12 weeks of cultivation the molds are growing and in this media the mycelium is formed in the liquid. We separate the liquid and the mycelium. The liquid is directly filtrated in a biohazard surrounding with end filter 0.45 µm. This filtered liquid is dialysed with a volatile buffer, so after freeze drying a minimum amount of salts is available. The mycelium is extracted with a 1 % phenol buffer, extensively mixed and filtrated with an end filter of 0.45 µm. The liquid is dialysed against a volatile buffer and freeze dried.